""" ================================================= Bootstrap and Closest Peak Direction Getters Example ================================================= This example shows how choices in direction-getter impact fiber tracking results by demonstrating the bootstrap direction getter (a type of probabilistic tracking, as described in [Berman2008]_) and the closest peak direction getter (a type of deterministic tracking). (Amirbekian, PhD thesis, 2016) Let's load the necessary modules for executing this tutorial. """ from dipy.data import read_stanford_labels from dipy.tracking import utils from dipy.tracking.local import (ThresholdTissueClassifier, LocalTracking) from dipy.io.streamline import save_trk from dipy.viz import window, actor, colormap as cmap renderer = window.Renderer() """ Now we import the CSD model """ from dipy.reconst.csdeconv import ConstrainedSphericalDeconvModel """ First we load our images and establish seeds. See the Introduction to Basic Tracking tutorial for more background on these steps. """ hardi_img, gtab, labels_img = read_stanford_labels() data = hardi_img.get_data() labels = labels_img.get_data() affine = hardi_img.affine seed_mask = labels == 2 white_matter = (labels == 1) | (labels == 2) seeds = utils.seeds_from_mask(seed_mask, density=1, affine=affine) """ Next, we fit the CSD model """ csd_model = ConstrainedSphericalDeconvModel(gtab, None, sh_order=6) csd_fit = csd_model.fit(data, mask=white_matter) """ we use the CSA fit to calculate GFA, which will serve as our tissue classifier """ from dipy.reconst.shm import CsaOdfModel csa_model = CsaOdfModel(gtab, sh_order=6) gfa = csa_model.fit(data, mask=white_matter).gfa classifier = ThresholdTissueClassifier(gfa, .25) """ Next, we need to set up our two direction getters """ """ Example #1: Bootstrap direction getter with CSD Model """ from dipy.direction import BootDirectionGetter from dipy.tracking.streamline import Streamlines from dipy.data import small_sphere boot_dg_csd = BootDirectionGetter.from_data(data, csd_model, max_angle=30., sphere=small_sphere) boot_streamline_generator = LocalTracking(boot_dg_csd, classifier, seeds, affine, step_size=.5) streamlines = Streamlines(boot_streamline_generator) renderer.clear() renderer.add(actor.line(streamlines, cmap.line_colors(streamlines))) window.record(renderer, out_path='bootstrap_dg_CSD.png', size=(600, 600)) """ .. figure:: bootstrap_dg_CSD.png :align: center **Corpus Callosum Bootstrap Probabilistic Direction Getter** We have created a bootstrapped probabilistic set of streamlines. If you repeat the fiber tracking (keeping all inputs the same) you will NOT get exactly the same set of streamlines. We can save the streamlines as a Trackvis file so it can be loaded into other software for visualization or further analysis. """ save_trk("bootstrap_dg_CSD.trk", streamlines, affine, labels.shape) """ Example #2: Closest peak direction getter with CSD Model """ from dipy.direction import ClosestPeakDirectionGetter pmf = csd_fit.odf(small_sphere).clip(min=0) peak_dg = ClosestPeakDirectionGetter.from_pmf(pmf, max_angle=30., sphere=small_sphere) peak_streamline_generator = LocalTracking(peak_dg, classifier, seeds, affine, step_size=.5) streamlines = Streamlines(peak_streamline_generator) renderer.clear() renderer.add(actor.line(streamlines, cmap.line_colors(streamlines))) window.record(renderer, out_path='closest_peak_dg_CSD.png', size=(600, 600)) """ .. figure:: closest_peak_dg_CSD.png :align: center **Corpus Callosum Closest Peak Deterministic Direction Getter** We have created a set of streamlines using the closest peak direction getter, which is a type of deterministic tracking. If you repeat the fiber tracking (keeping all inputs the same) you will get exactly the same set of streamlines. We can save the streamlines as a Trackvis file so it can be loaded into other software for visualization or further analysis. """ save_trk("closest_peak_dg_CSD.trk", streamlines, affine, labels.shape) """ .. [Berman2008] Berman, J. et al., Probabilistic streamline q-ball tractography using the residual bootstrap, NeuroImage, vol 39, no 1, 2008 .. include:: ../links_names.inc """